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Antibodies sure to the blocking peptide no more bind towards the epitope over the focus on protein. By comparing the staining through the blocked antibody versus the antibody by itself, you can see which staining is specific.
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This information outlines further doable optimization of cellular and immunoassay parameters to be sure the very best final results are...
This guideline outlines more doable optimization of mobile and immunoassay parameters to make certain the absolute best success are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide generally consists of the epitope recognized from the antibody.
Target Information Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital job in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two added splice variants were documented but in-depth nucleotide sequence info will not be yet offered.
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